B-hGHRL mice
| Strain Name | C57BL/6-Ghrltm1(GHRL)Bcgen/Bcgen | Common Name | B-hGHRL mice |
| Background | C57BL/6N | Catalog number |
110760 |
| Aliases | MTLRP | ||
模型验证
Protein expression analysis
mRNA expression analysis
Strain specific Obestatin & Ghrelin expression analysis in homozygous B-hGHRL mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hGHRL mice (H/H), and analyzed by ELISA with Obestatin & Ghrelin ELISA kit. Obestatin was detectable both in wild-type mice and homozygous B-hGHRL mice, as the ELISA kit is cross-recognize both human and mouse Obestatin. Human Ghrelin was detected in homozygous B-hGHRL mice but not in wild-type mice.
Strain specific analysis of GHRL mRNA expression in wild-type C57BL/6 mice and B-hGHRL mice by RT-PCR. Brain RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGHRL mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human GHRL primers. Human GHRL mRNA was detected in homozygous B-hGHRL mice but not in wild-type mice.
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